Lobe
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Petal
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What is GradientEZ?
GradientEZ is a qualitative cell migration and invasion assay that enables cells to migrate in three dimensions as they do in living tissues. It is straightforward to use and works like a piece of paper. Simply, pipet cells in the center and add test agents to petals or lobes to model physiologically relevant, gradient-dependent pathological conditions in 3D. Alternatively, add invasive cancer cells to the center and normal cells to lobes.
Fluorescently labeled cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell migration studies using fluorescence microscopy.
Suitable for both adherent and suspension cells.
Fluorescently labeled cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell migration studies using fluorescence microscopy.
Suitable for both adherent and suspension cells.
How it works:
Method 1:Migrating or invading cells, and different cell populations or test compounds may be added to the center and 6 petals or lobes
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Method 2:To test metastatic potential of cancer cells in vitro, add invasive cells to the center and normal cells (e.g. brain, lung, liver, bone, adrenal, etc.) to lobes and track invasion to begin to elucidate where they might metastasize in vivo
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Benefits of using GradientEZ
• Closely mimics physiologically relevant in vivo conditions
• Fits in any Petri dish, 6-well to 12-well plates & 6-well inserts
• 6-petal format fits in 6-well to 24-well plates
• Test modulators of cell motility or invasion, in parallel, in one assay
• Setup complex gradients
• Use an inhibitor & inducer on opposing petals or lobes, to direct the cells more efficiently, as in vivo
• Multiple agent testing in the same well
• Up to six different concentrations of the same agent can be tested in one well
• Fits in any Petri dish, 6-well to 12-well plates & 6-well inserts
• 6-petal format fits in 6-well to 24-well plates
• Test modulators of cell motility or invasion, in parallel, in one assay
• Setup complex gradients
• Use an inhibitor & inducer on opposing petals or lobes, to direct the cells more efficiently, as in vivo
• Multiple agent testing in the same well
• Up to six different concentrations of the same agent can be tested in one well