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3D Cell-based assays

Assays routinely used in our laboratory with SeedEZ and Perfused Organ Panel

​Unless labeled “terminal,” all assays are mix-and-measure assays done by:

     A.  adding assay reagents to live cells, followed by collection of supernatants, or
     B.  assaying the culture medium directly for analytes consumed (e.g. glucose) or released (e.g. cytokines) by cells
Cell health can be monitored over time using method 
B by assaying medium when it is exchanged.
All assays are quantified using a plate reader (bioluminescence, fluorescence, or absorbance readout).
Some assays may be multiplexed and run in the same well. For example, bioluminescence and fluorescence assays.
Cell Viability and Apoptosis
​Measure
Method
Cell Titer Glo
ATP (terminal)
A
Cell Titer Blue
Live Cell Number (standard curve)
A
alamarBlue
Live Cell Number (standard curve)
A
CCK-8
Live Cell Number (standard curve)
A
LDH Release
Cell Death (toxicity)
B
Lysed LDH
Live Cell Number (terminal)
A
Live/Dead Staining
Calcein AM/ Propidium Iodide
Imaging, fluorescence microscopy
Caspase-3
Terminal
A
CytoTox Fluor
Cell Death (toxicity)
A
Functional Assays
Measure
Method
Glucose Glo
Glucose Consumption
​B
EnzyChrom Glucose
Glucose Consumption
B
L-Lactate
Lactate Production
B
QuantiChrom Urea
Urea Synthesis
B
Bile Acids
Terminal
A
Amplex Red AChE/ACh
Acetylcholinesterase activity/Acetylcholine content
A
Acetylthiocholine (ATCh) and DTNB (Ellman's reagent)
Acetylcholinesterase activity
A
Butrylthiocholine (BuTCh) and DTNB (Ellman's Reagent)
Butyrylcholinesterase activity
A
Alanine Aminotransferase (ALT)
​ALT Enzyme Activity (terminal)
A
Drug Metabolism
Measure
Method
P450-Glo Assays
CYP3A4, CYP1A2, CYP2C9, CYP2B6 (luminescent)
A
EROD (ethoxyresorufin-O-deethylation)
CYP1A Assay (fluorometric)
A
MROD (methoxyresorufin-O-demethylation)
CYP1A2 Assay (fluorometric)
A
​3-cyano-7-ethoxycoumarin
​​CYP2C19 Assay (fluorometric)
A
Stress and Inflammation
Measure
Method
Griess Assay
RNS production (nitrite; indirect nitric oxide)
B
TNFalpha ELISA
B
CXCL10 ELISA
B
Luminex Inflammation Panel
B
Amplex Red
hydrogen peroxide (indirect ROS production)
B
Glutathione (GSH/GSSG)
Terminal
A
TBARS
Lipid Peroxidation (terminal)
A
Protein
Measure
Method
Immunofluorescence
Directly in scaffold (terminal)
A
Western Blot
Media samples, or Protein extraction directly from cells in scaffold (terminal)
A, B
ELISA
Media samples, or Protein extraction directly from cells in scaffold (terminal)
A, B
Nucleic Acid
Measure
Method
DNA/RNA extraction for qPCR
Terminal
A
​
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  • Home
  • Company
    • About us
    • Partners >
      • New Partnerships
    • Careers
    • News >
      • Press Dr. Eckhard von Keutz
    • Events
  • Technology
    • Overview
    • Science >
      • Why 3D?
      • Our 3D models
      • Cell Types
      • Assays
    • Perfused Organ Panel
    • SeedEZ
    • Blood Substitute
    • GradientEZ
    • Publications
    • Featured publication
  • Shop
    • Services >
      • Liver toxicity service
      • Mitochondrial toxicity service
    • Products >
      • Perfused Organ Panel microphysiological system
      • SeedEZ 3D cell culture scaffold
      • GradientEZ 3D cell invasion assay
    • Distributors
  • Support
    • Instructions
    • Cell Types
    • 3D cell-based assays
    • Applications
    • FAQs
    • Contact us