LENA BIOSCIENCES
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​​3D Cell Culture



​Culturing cells in a 3D environment restores
cell morphology, receptor distribution,​
​and normal cell functions

BEATING CARDIOMYOCYTES CULTURED IN SEEDEZ

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C. M. Didier, A. Kundu, J. T. Shoemaker, J. Vukasinovic and S. Rajaraman. 
​
SeedEZTM Interdigitated Electrodes and Multifunctional Layered Biosensor Composites (MLBCs): A Paradigm Shift in the Development of In Vitro BioMicrosystems. Journal of Microelectromechanical Systems. 2020. With permission.
DOI: 10.1109/JMEMS.2020.3003452 | https://ieeexplore.ieee.org/document/9126794
In the last decade, three dimensional (3D) cell culture has become an increasingly popular research tool used in labs worldwide.  When grown in three dimensions, cells are no longer restricted to growing within a monolayer, and can begin to adhere to each other and the extracellular matrix, mimicking the native microenvironment from which the cells originated.  The architecture of our SeedEZ scaffold allows cells to revert to their natural morphology.  The advantages to 3D cell culture are expansive, allowing cells to express physiological proportions and localization of receptors, and respond to stimuli in the way that is naturally observed.
​

Standard 2D plating method, using a Petri dish or a multi-well plate, is still the most common practice for culturing cell lines.  When using the canonical 2D method, we see an unnatural morphology in cells.  The cells are flat and adhered to the rigid plastic surface.  The 2D method results in altered cell cytoskeleton and produces a monolayer of cells without the same cell-cell communication or morphology seen biologically.
We are excited to be a part of this new front.
Thank you for your trust and confidence in the data discovered using our systems.
Find Out Which Cells We've Used

Other Culture Solutions

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Method: 2D Culture
​
Limitations:
  • Contact inhibition​
  • Loss of function
  • Studies limited to 4-7 days
  • Cannot test multiple drug doses
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Method: 3D Spheroid

​Limitations:
  • ​Avascular Tumors
  • For normal tissues: hypoxic & necrotic core
  • ​Delicate handling
  • May be aspirated in media changes
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Method: ECM Co-Culture
​
Limitations:
  • Inconsistent shape
  • Culture-to-culture variability
  • Poor long-term adhesion
  • Easy to aspirate gel in media changes
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Method: Organ-on-a-Chip

​Limitations:
  • One sample
  • Microchannels clog
  • Bubbles interrupt flow
  • Drugs are adsorbed
  • Flow washes away cell signaling

Our Solution

Our 3D system, Perfused Organ Panel (POP) is a three-part solution consisting of the PerfusionPal plate, SeedEZ, and Blood Substitute, resulting in a uniquely accessible organo-mimetic 3D platform that provides perfusion and superior gas delivery to cells.

Perfused Organ Panel

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SeedEZ Scaffolds

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Blood Substitute

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​​Learn about 3D cell culture and Perfused Organ Panel technology
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  • Home
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      • Why 3D?
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    • Perfused Organ Panel
    • SeedEZ
    • GradientEZ
    • Blood Substitute
    • Publications
  • Shop
    • Products >
      • Perfused Organ Panel
      • SeedEZ
      • GradientEZ
    • Services
    • Distributors
  • Support
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    • Downloads
    • FAQs
    • Cell Types
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    • Contact Us