BEATING CARDIOMYOCYTES CULTURED IN SEEDEZ
C. Didier, A. Kundu, J. Shoemaker, J. Vukasinovic and S. Rajaraman, “SeedEZ Interdigitated Electrodes and Multifunctional Layered Biosensor Composites: A Paradigm Shift in the Development of In Vitro Biomicrosystems”, the 19th Solid State Sensors, Actuators and Microsystems Workshop. With permission.
In the last decade, three dimensional (3D) cell culture has become an increasingly popular research tool used in labs worldwide. When grown in three dimensions, cells are no longer restricted to growing within a monolayer, and can begin to adhere to each other and the extracellular matrix, mimicking the native microenvironment from which the cells originated. The architecture of our SeedEZ scaffold allows cells to revert to their natural morphology. The advantages to 3D cell culture are expansive, allowing cells to express physiological proportions and localization of receptors, and respond to stimuli in the way that is naturally observed.
Standard 2D plating methods, using a Petri dish or multi-well plate, is still the most common practice for culturing cell lines. When using the canonical 2D method, we see an unnatural morphology in cells. The cells are flat and adhered to the rigid plastic surface. The 2D method results in altered cell cytoskeleton and produces a monolayer of cells without the same cell-cell communication or morphology seen biologically.
Standard 2D plating methods, using a Petri dish or multi-well plate, is still the most common practice for culturing cell lines. When using the canonical 2D method, we see an unnatural morphology in cells. The cells are flat and adhered to the rigid plastic surface. The 2D method results in altered cell cytoskeleton and produces a monolayer of cells without the same cell-cell communication or morphology seen biologically.
Other Culture Solutions
Method: 2D Culture
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Method: 3D Spheroid
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Method: ECM Co-Culture
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Method: Organ-on-a-Chip
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Our Solution
Our 3D system, Perfused Organ Panel (POP) is a three-part solution consisting of the PerfusionPal plate, SeedEZ, and Blood Substitute, resulting in a uniquely accessible organo-mimetic 3D platform that provides perfusion and superior gas delivery to cells.
Perfused Organ Panel
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SeedEZ Scaffolds
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Blood Substitute
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