Culturing cells in a 3D environment
restores cell morphology, receptor distribution and normal cell functions
With permission. O’Driscoll, L. The Relevance of using 3D cell cultures, in addition to 2D monolayer cultures, when evaluating breast cancer drug sensitivity and resistance. Oncotarget, 2016.
In the last decade, three dimensional (3D) cell culture has become an increasingly popular research tool used in labs worldwide. When grown in three dimensions, cells are no longer restricted to growing within a monolayer, and can begin to adhere to each other and the extracellular matrix, mimicking the native microenvironment from which the cells originated. The architecture of our SeedEZ scaffold allows cells to revert to their natural morphology. The advantages to 3D cell culture are expansive, allowing cells to express physiological proportions and localization of receptors, and respond to stimuli in the way that is naturally observed.
Standard 2D plating methods, using a Petri dish or multi-well plate, is still the most common practice for culturing cell lines. When using the canonical 2D method, we see an unnatural morphology in cells. The cells are flat and adhered to the rigid plastic surface. The 2D method results in altered cell cytoskeleton and produces a monolayer of cells without the same cell-cell communication or morphology seen biologically.
Standard 2D plating methods, using a Petri dish or multi-well plate, is still the most common practice for culturing cell lines. When using the canonical 2D method, we see an unnatural morphology in cells. The cells are flat and adhered to the rigid plastic surface. The 2D method results in altered cell cytoskeleton and produces a monolayer of cells without the same cell-cell communication or morphology seen biologically.
Other Culture Solutions
Method: 2D Culture
Limitations:
|
Method: 3D Spheroid
Limitations:
|
Method: ECM Co-Culture
Limitations:
|
Method: Organ-on-a-Chip
Limitations:
|
Our Solution
Our 3D system, Perfused Organ Panel (POP) is a three-part solution consisting of the PerfusionPal plate, SeedEZ, and Blood Substitute, resulting in a uniquely accessible organo-mimetic 3D platform.
Perfused Organ Panel
|
SeedEZ Scaffolds
|
Blood Substitute
|