Our 3D Models
Human liver model
Increased CYP activity in Induced Primary Human Hepatocytes cultured with PerfusionPal
Phase 1 Drug Metabolism in primary human hepatocytes (BioIVT Cryoplateable 10-donor Liverpool) was measured over a 7 day period. Cells were cultured in either a 2D format using a multi-well plate, or in the Lena Biosciences PerfusionPal system (n=3 for all conditions). At day 6, cells were induced with either Rifampin (10 uM) or Omeprazole (50 mM) and data was collected on day 7. Perfusion rate for the PerfusionPal data corresponds to 16 volume changes per 24 hour period. All data was normalized by their respective un-induced data (shown in black and green). Asterisk indicate significant enhancement of induced hepatocytes in the PerfusionPal system when compared to the control. All induced data was normalized to baseline CYP activity *p<0.05, **p<0.01, ***p<0.001. |
Baseline CYP Activity in Primary Human Hepatocytes after 7 days in culture
Baseline metabolism was recorded for various CYP enzymes in primary human hepatocytes (BioIVT Cryoplateable 10-donor Liverpool) in cells cultured in 2D or PerfusionPal. Asterisk indicate significant difference in metabolic activity due to plating condition. Cells cultured in the Lena Biosciences PerfusionPal system show an enhanced CYP activity compared to the control. All values were normalized to the 2D control. Note: Log Scale for baseline activity graph. *p<0.05, **p<0.01, ***p<0.001. |
Human lung model
Patient-derived NSCLC cells plated on Blood Substitute form a more viable, tissue-like 3D construct Patient-derived NSCLC cells were plated either atop the Blood substitute with medium additive, or in a multi-well plate at one order of magnitude higher density (n=6 per condition). On day 7, cells on the Blood Substitute were noticeably denser, exhibiting a tissue-like appearance (left). Total cellular respiration on day 10, as measured by alamarBlue, showed that the cells on the Blood Substitute were three times more metabolically active than those in the multi-well plate. LDH release was twice as high in cells plated without Blood substitute, indicating higher viability using the blood substitute. **p<0.01, ***p<0.001 |
Blood substitute increases cell survival over time and limits overgrowth of cells H1299 NSCLC cells were grown in a multi-well plate or atop the Blood Substitute in 6-well inserts. Photos were acquired at the time points indicated. On days 2 and 5, cell morphology and distribution were comparable. At day 8, the cells in the multi-well plate had become overgrown and many appeared to be dead. The Blood Substitute may limit this excessive growth through the more physiological delivery of gases such as oxygen. |
Human brain model
3D Cortical Tri-Culture Model
Triculture model of iPSC neurons, astrocytes and HMC3 microglia seeded in SeedEZ scaffolds and grown in one of 3 conditions. Scaffolds were stained using calcein AM.
After 2 weeks in culture, media from iPSC neurons, iPSC astrocytes, and HMC3 microglia was sampled to detect off-target toxicity of activated T-cell medium using alamarBlue. Cells were grown in static conditions (blood substitute, without perfusion) or in Perfused conditions. Control conditions consisted of the triculture seeded in SeedEZ without blood substitute or perfusion.
After 2 weeks in culture, media from iPSC neurons, iPSC astrocytes, and HMC3 microglia was sampled to detect off-target toxicity of activated T-cell medium using alamarBlue. Cells were grown in static conditions (blood substitute, without perfusion) or in Perfused conditions. Control conditions consisted of the triculture seeded in SeedEZ without blood substitute or perfusion.