Lena Biosciences offers in vitro services using SeedEZ, Perfused Organ Panel products, and ultra-low attachment plates.
Typical models: liver, brain and tumors. T cells can be included with the models.
Typical models: liver, brain and tumors. T cells can be included with the models.
3D Cell-Based Assays
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We routinely run assays with primary cells and iPSC-derived cells.
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Organ-on-a-Chip
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Studies are run in our PerfusionPal platform that enables parallel testing of up to 48 statistically-independent organ cultures in one plate.
Models offered: liver, brain, and tumors. We can include T cells. |
Organoids
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Organoids can be cultured and tested in our proprietary PerfusionPal organ-on-a-chip-like system, or in ultra-low attachment plates. They can be formed from iPSCs or other cells of interest. We can also grow organoids from tissue samples.
Models offered: liver, brain, and tumors. |
3D Cell Culture Models
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We generate advanced 3D tissue models using co-cultures of primary cells or cell lines based on your specifications, or using cells provided by you. Models can be cultured in 3D in SeedEZ, as spheroids, or perfused in PerfusionPal.
Models offered: liver, brain, and tumors. |
Neuronal Assays
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Neuronal assays are run in 2D and in 3D in SeedEZ. Testing may also be performed in our cortical model comprising tri-culture of neurons, astrocytes and microglia. Cells may be further supported by our blood substitute that increases cellular respiratory metabolism several-fold and reduces cell death. Cultures can also be perfused in our proprietary PerfusionPal organ-on-a-chip-like system providing month-long support. Cell types: iPSCs or rat, or mouse primary cells.
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ADME services, CYP450 Induction and Inhibition
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Phase I drug metabolism services are performed using perfused 3D cultures of primary human hepatocytes with log-fold increase in CYP450 activity. CYP450 assays are run using primary human hepatocytes, or differentiated NoSpin HepaRG cells. Assays: CYP450-Glo assays multiplexed with fluorescence assays like EROD.
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In vitro Hepatotoxicity Testing
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Short-term and long-term intrinsic DILI using primary liver cells with high oxidative metabolism and CYP monooxygenase activity in Perfused Organ Panel. If Phase I metabolite does not appear to be toxic, and no Phase III toxicity is found, we use other liver models to confirm potential Phase II toxicity.
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Cytotoxicity and In Vitro Toxicity Assays
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Toxicity assays are offered in 2D and in 3D , in our SeedEZ scaffold and Perfused Organ Panel. Assays may be performed using a client's cells and standard assays (CellTiter-Glo or total LDH, kinetic LDH release, CCK-8, AlamarBlue etc.) For immune cell therapy toxicity, immune cells may be co-cultured with the organ models, or the organ models exposed to activated immune cell medium to assess toxicity in vitro.
Models offered: liver, brain, and tumors. |
Off-Target Drug Effects
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Off-target toxicity is evaluated in SeedEZ or Perfused Organ Panel. For in vitro cell therapy testing, the toxicity of activated immune cells is measured after co-culture with 3D human organ models, or after incubation of 3D organ models in activated immune cell medium.
Models offered: liver, brain, and tumors. |
Cell and Tissue Culture
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We routinely culture in 2D and 3D. We can support 3D cell cultures (SeedEZ), spheroids in ULA plates, and perfused 3D cell cultures in our Perfused Panel (SeedEZ), tissue slices and organoids.
Models offered: liver, brain, and tumors. |
Induced Pluripotent Stem Cell (iPSC) Culture
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Routine 2D and 3D iPSC cell cultures and co-cultures.
Models offered: brain and liver. |