Lena Biosciences offers in vitro services using SeedEZ and PerfusionPal products
3D Cell-Based Assays
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The assays are run in our proprietary SeedEZ scaffold that enables both cadherine and integrine signaling in 3D. Common assays include: CellTiter-Glo, alamarBlue, LDH release, etc. We routinely run assays with primary cells and iPSCs and have extensive experience with brain co-cultures, liver, cancers such as breast, liver, lung etc.
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Organ-on-a-Chip
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Studies are run in our PerfusionPal platform that enables parallel testing of up to 48 statistically-independent organ cultures in one plate.
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Organoids
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Organoids are formed using co-cultures of iPSCs and primary cells and cultured in our proprietary PerfusionPal organ-on-a-chip-like system.
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3D Cell Culture Models
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We generate advanced 3D tissue models using co-cultures of primary cells or cell lines based on your specifications, or using cells provided by you. Models may be perfused (interstitial perfusion) and vascularized in our proprietary PerfusionPal system to model vascularized tumors, for example.
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Neuronal Assays
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Neuronal assays are run in 2D and 3D. Testing may also be performed in our cortical model comprising tri-culture of neurons, astrocytes and microglia. Cells may be further supported by our blood substitute that increases cellular respiratory metabolism several-fold and reduces cell death. Cultures can also be perfused in our proprietary PerfusionPal organ-on-a-chip-like system providing month-long support.
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ADME services, CYP450 Induction and Inhibition
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Phase I drug metabolism services are performed using perfused 3D cultures of primary human hepatocytes with log-fold increase in CYP450 activity. CYP450 assays are run using primary human hepatocytes in 2D and 3D.
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In vitro Hepatotoxicity Testing
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Short-term and long-term DILI using primary liver cells with log-fold increase in Phase I drug metabolism and multifold increase in cell respiratory metabolism.
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Cytotoxicity and In Vitro Toxicity Assays
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Toxicity assays are offered in 2D and 3D, and in advanced, synthetically vascularized models supported by blood substitute and interstitial perfusion. Assays may be performed using a client's cells and standard assays (CellTiter-Glo or total LDH, kinetic LDH release etc.) For immune cell therapy toxicity, immune cells may be co-cultured with the organ models, or the organ models exposed to activated immune cell medium to assess toxicity in vitro.
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Off-Target Drug Effects
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Off-target toxicity is evaluated in our 12-well organ-on-a-chip-like system PerfusionPal. For in vitro cell therapy testing the toxicity of activated immune cells is measured after co-culture with 3D human organ models, or after incubation of 3D organ models in activated immune cell medium.
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Cell and Tissue Culture
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We routinely culture in 2D and 3D, and generate organoid-like and perfused 3D cultures. Using our proprietary Blood Substitute and/or perfusion we can support tissue slice cultures longer.
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Induced Pluripotent Stem Cell (iPSC) Culture
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Routine 2D and 3D iPSC cell cultures and co-cultures. 3D cell cultures can also be perfused in our proprietary PerfusionPal system.
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