95% of cancer drugs fail in human trials
after being tested in animals
Prof. Valerie Speirs and the colleagues at the University of Aberdeen, UK developed a fully humanized, 3D perfused breast cancer model. In a study published in PLoS One, they show how perfusion and hemoglobin-like oxygen delivery influence breast cancer (BC) growth and responses to a commonly used drug, tamoxifen:
Validation of a 3D perfused cell culture platform as a tool for humanised preclinical drug testing in breast cancer using established cell lines and patient-derived tissues
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283044
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283044
Patient-derived, ER-positive breast cancer models are difficult to establish in mice. Typically, the engraftment rate is lower than 15% which limits the number of models that can be used in research.
Perfused Organ Panel successfully supported the growth of all tested primary BC tissues and enabled long-term drug testing in vitro. Perfused tissues had higher metabolic activity, ranging from 2.3-fold (case 2724) to 1.3-fold (case 1756) compared to static BC cultures. Tumor heterogeneity between the patient samples leads to patient- and sample-specific drug responses which were captured under perfused conditions.
Most tumors bigger than 1-2 mm in diameter are perfused with blood which provides nutrients and oxygen required for tumor growth and metastasis. Perfused tumors in vitro may be able to more accurately model a tumor which the patient has at a time of drug treatment. A non-perfused tumor in vitro models an earlier disease stage, a tumor which the patient had when the tumor formed.
Perfused Organ Panel successfully supported the growth of all tested primary BC tissues and enabled long-term drug testing in vitro. Perfused tissues had higher metabolic activity, ranging from 2.3-fold (case 2724) to 1.3-fold (case 1756) compared to static BC cultures. Tumor heterogeneity between the patient samples leads to patient- and sample-specific drug responses which were captured under perfused conditions.
Most tumors bigger than 1-2 mm in diameter are perfused with blood which provides nutrients and oxygen required for tumor growth and metastasis. Perfused tumors in vitro may be able to more accurately model a tumor which the patient has at a time of drug treatment. A non-perfused tumor in vitro models an earlier disease stage, a tumor which the patient had when the tumor formed.
Our revolutionary Perfused Organ Panel
models vascularized and perfused tumors
that patients have at the time of drug treatment
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models vascularized and perfused tumors
that patients have at the time of drug treatment
Read the article
