95% of cancer drugs fail in human trials
after being tested in animals
Prof. Valerie Speirs and the colleagues at the University of Aberdeen, UK developed a fully humanized, 3D perfused breast cancer model. In a study published in PLoS One, they show how perfusion and hemoglobin-like oxygen delivery influence breast cancer (BC) growth and responses to a commonly used drug, tamoxifen:
Validation of a 3D perfused cell culture platform as a tool for humanised preclinical drug testing in breast cancer using established cell lines and patient-derived tissues
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283044
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283044
Patient-derived, ER-positive breast cancer models are difficult to establish in mice. Typically, the engraftment rate is lower than 15% which limits the number of models that can be used in research.
Perfused Organ Panel successfully supported the growth of all tested primary BC tissues and enabled long-term drug testing in vitro. Perfused tissues had higher metabolic activity, ranging from 2.3-fold (case 2724) to 1.3-fold (case 1756) compared to static BC cultures. Tumor heterogeneity between the patient samples leads to patient- and sample-specific drug responses which were captured under perfused conditions.
Most tumors bigger than 1-2 mm in diameter are perfused with blood which provides nutrients and oxygen required for tumor growth and metastasis. Perfused tumors in vitro may be able to more accurately model a tumor which the patient has at a time of drug treatment. A non-perfused tumor in vitro models an earlier disease stage, a tumor which the patient had when the tumor formed.
Perfused Organ Panel successfully supported the growth of all tested primary BC tissues and enabled long-term drug testing in vitro. Perfused tissues had higher metabolic activity, ranging from 2.3-fold (case 2724) to 1.3-fold (case 1756) compared to static BC cultures. Tumor heterogeneity between the patient samples leads to patient- and sample-specific drug responses which were captured under perfused conditions.
Most tumors bigger than 1-2 mm in diameter are perfused with blood which provides nutrients and oxygen required for tumor growth and metastasis. Perfused tumors in vitro may be able to more accurately model a tumor which the patient has at a time of drug treatment. A non-perfused tumor in vitro models an earlier disease stage, a tumor which the patient had when the tumor formed.
Our revolutionary Perfused Organ Panel
models vascularized and perfused tumors that patients have at the time of drug treatment
Read the article
models vascularized and perfused tumors that patients have at the time of drug treatment
Read the article
|